Sophie Guillemin was born in Paris. She received her BAC (French academic qualification) in 1996. Before making her film debut with Ennui, L' (1998) in 1998 she spent some time working at Disneyland Paris, as well as being a waitress at Quick.
She was nominated for Cesars for her roles in both L'Ennui and Harry, un ami qui vous veut du bien (2000).